Coding

Part:BBa_K129001:Design

Designed by: Burak YILMAZ   Group: iGEM08_METU_Turkey   (2008-11-04)


Htdr-deltarhodopsin-light driven pump


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 775
    Illegal PstI site found at 789
    Illegal NotI site found at 7
    Illegal NotI site found at 782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 610
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 775
    Illegal PstI site found at 789
    Illegal AgeI site found at 468
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Homology between Bbacteriorhodopsin (bR) expressed in purple membrane of Halobacterium salinarum is as low as 53%.


Source

sequenced a dR homologue from Haloterrigena turkmenica (JCM9743)GenBank Accession No. AB009620

References